Peptide Reconstitution Calculator
Calculate concentration, volume and graduated units for lyophilised peptides. For in vitro laboratory research use only.
Worked Reconstitution Examples
Real examples using common vial sizes. All figures assume an insulin syringe where 100 units = 1 mL (so 1 unit = 0.01 mL). For research use only.
BPC-157 10mg + 2 mL BAC water
Concentration: 5 mg/mL · 1 unit = 0.05 mg (50 mcg) · 10 units (0.1 mL) = 0.5 mg
HGH Fragment 176-191 5mg + 2 mL BAC water
Concentration: 2.5 mg/mL · 1 unit = 0.025 mg (25 mcg) · 10 units (0.1 mL) = 0.25 mg (250 mcg)
TB-500 10mg + 2 mL BAC water
Concentration: 5 mg/mL · 1 unit = 0.05 mg (50 mcg) · 10 units (0.1 mL) = 0.5 mg
Glow Stack 70mg (blend) + 2.5 mL BAC water
A blend contains multiple peptides in one vial, so each is calculated separately from its own share of the total. Glow Stack is GHK-Cu 50mg + BPC-157 10mg + TB-500 10mg.
Drawing 10 units (0.1 mL) gives all three at once:
- GHK-Cu (20 mg/mL) → 2.0 mg
- BPC-157 (4 mg/mL) → 0.4 mg
- TB-500 (4 mg/mL) → 0.4 mg
GHK-Cu 100mg + 2 mL BAC water
Concentration: 50 mg/mL · 1 unit = 0.5 mg · 10 units (0.1 mL) = 5 mg. A larger reconstitution volume can be used if smaller, easier-to-measure draws are preferred.
NAD+ 500mg + 5 mL BAC water
Concentration: 100 mg/mL · 1 unit = 1 mg · 10 units (0.1 mL) = 10 mg
Frequently Asked Questions
How do I calculate peptide concentration?
Divide the peptide amount (mg) by the volume of bacteriostatic water added (mL). For example, 10mg in 2mL gives 5mg/mL.
What does one unit on an insulin syringe equal?
On a standard U-100 insulin syringe, 100 units = 1mL, so 1 unit = 0.01mL. Multiply your concentration (mg/mL) by 0.01 to find the mg per unit.
How do I calculate a peptide blend?
Calculate each peptide in the blend separately using its own mg amount, then divide by the shared reconstitution volume. Each component has its own concentration, but they are all drawn together in the same volume.
What water is used to reconstitute research peptides?
Bacteriostatic water (sterile water with 0.9% benzyl alcohol) is the standard solvent for reconstituting lyophilised research peptides, as the preservative allows multi-use over several weeks under refrigeration.
All calculations and examples are provided for laboratory research purposes only. Revial Labs products are not for human consumption.
Understanding Peptide Blends
Single-peptide vials are straightforward: divide the milligrams by the millilitres of bacteriostatic water to get the concentration. Blends are where reconstitution maths is most often misunderstood. In a blend, the stated total milligrams are split across several peptides in one vial, so each component has its own concentration, yet all are drawn together in the same volume.
Take the Glow Stack as an example: its 70 mg total is GHK-Cu 50 mg, BPC-157 10 mg, and TB-500 10 mg. Reconstituted in 2.5 mL, a single 10-unit (0.1 mL) draw contains, simultaneously, GHK-Cu 2 mg, BPC-157 0.4 mg, and TB-500 0.4 mg — three different amounts from one draw. The calculator above and the example cards handle this automatically, but it helps to understand why the numbers differ.
Frequently Asked Questions
How do I calculate peptide concentration?
Divide the total peptide mass (mg) by the volume of bacteriostatic water added (mL). For example, 10 mg in 2 mL gives 5 mg/mL. The calculator above does this automatically and also converts to a per-unit draw on an insulin syringe.
What is an insulin unit in mL?
On a standard U-100 insulin syringe, 100 units equals 1 mL, so 1 unit is 0.01 mL and 10 units is 0.1 mL.
How do I calculate a peptide blend?
Calculate each component separately against the same reconstitution volume, because every peptide in the vial shares that single volume. As shown with the Glow Stack above, one blend gives different concentrations for each of its peptides, all drawn together.
What water is used to reconstitute research peptides?
Bacteriostatic water (sterile water with 0.9% benzyl alcohol) is the standard solvent for reconstituting lyophilised research peptides, as the preservative allows multi-draw use over time under refrigeration. See Bacteriostatic Water.
Does more water change the amount of peptide?
No — the total peptide mass in the vial is fixed. Adding more water lowers the concentration (mg/mL), which means a larger draw volume for the same mass. This can make very small target amounts easier to measure accurately on a syringe.
This calculator is provided for research reference only. Revial Labs products are supplied strictly for laboratory research use and are not for human consumption.